Alex Oxlade-Chamberlain is anxiously awaiting the results of a scan that could spell the end of his World Cup.

The England forward sustained a knee injury in the 2-2 draw against Ecuador. He was forced off in the 64th minute after a heavy challenge from Carlos Gruezo and will have a scan on Thursday to determine whether he has sustained ligament damage, which would rule him out of the tournament in Brazil.

The friendly game was Oxlade-Chamberlain’s first appearance since April 20 after a groin injury had ruled him out of the end of the Premier League season and threatened his World Cup spot.

Raheem Sterling’s hopes of forcing his way into the starting line-up for the Group D opener against Italy have also been undermined after he was sent off for a foul on Manchester United’s Antonio Valencia. Fifa confirmed that the Liverpool winger will be ­suspensed for the final World Cup warm-up game against Honduras on Saturday.

Roy Hodgson, the England manager, said: “Oxlade-Chamberlain is the one that bothers us. His knee buckled under the challenge as the player turned into him. He’ll have a scan tomorrow, I’d imagine. When we’ve had the scan, we’ll know more. Jack Wilshere suffered from cramp, Luke Shaw too. We took them off for those reasons alone.”

Hodgson was unhappy with Valencia’s reaction to Sterling’s challenge. “It was a fierce challenge from young Raheem, not a foul challenge,” Hodgson said. “He played the ball and accelerated into the ball. I’m disappointed with Antonio’s reaction, grabbing the player. Had he not jumped up there wouldn’t have been a problem but, when he does that, the referee had to deal with that and sent Raheem off.